DNA Fingerprinting Lab

1. DNA is unique for everyone. The only exception is if a person has what?
An identical twin

2. What are DNA fingerprints used for?
Determining biological parents, or determining the suspect of a crime.

3. What “crime” was committed?
Someone licked a NOVA holographic lollipop.

4. What bodily fluid was removed from the “crime scene” to get DNA?
Saliva.

5. What does a restriction enzyme do?
They cut the long DNA molecules in certain places.

6. What is agarose gel?
It's a molecular strainer, allowing small pieces of DNA to move through.

7. What is electrophoresis?
A process that moves moleecules with an electric current.

8. Smaller fragments of DNA move ____________ than longer strands?
Easier

9. Why do you need to place a nylon membrane over the gel?
So the DNA can be separated from the gel.

10. Probes attach themselves to __________
DNA fragments.

11. Which chemical in your “virtual lab” is radioactive?
Probes.

12. Sketch your DNA fingerprint.

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13. Based on your DNA fingerprint, who licked the lollipop?
Honey Sweet

14. What kinds of things could you do at the DNA workshop?
Make new DNA strands, replicate DNA, or perform protien synthesis.

15. Whodunnit? Officials have sticky problem in Haiti Maggie Fox. November 18, 2010
The cholera outbreak in Haiti is currently being tracked. Suspects include Nepalese troopers from a recent UN mission.

Mitosis Simulation Questions

1. Which stage does the following occur
Chromatin condenses into chromosomes: prophase
Chromosomes align in center of cell: metaphase
Longest part of the cell cycle: interphase
Nuclear envelope breaks down: prometaphase
Cell is cleaved into two new daughter cells: cytokinesis
Daughter chromosomes arrive at the poles: telophase

2. More chromosome questions
How many chromosomes are visible at the beginning of mitosis? 23
How many are in eachdaughter cell at the end of mitosis? 46
The little green T shaped things on the cell are: centrioles
What happens to the centrioles during mitosis? spindle fibers grow on them.

3. Sketches
Prophase:
Metaphase:
Telophase:


4. Amount and percent of collected cells
Interphase: 20 (55%)
Prophase: 10 (28%)
Metaphase: 3 (8%)
Anaphase: 2 (6%)
Telophase: 1 (3%)
Total: 36 (100%)

5. Whitefish and onion views

Whitefish:
View 1: cytokinesis
View 2: metaphase
View 3: prophase
View 4: telophase

Onion:

View 1: metaphase
View 2: prophase
View 3: interphase
View 4: cytokinesis
View 5: cytokinesis